100 Single-Cell RNA Sequencing Is Revolutionizing Our Understanding Of Cancer While traditional gene expression analysis using RNA-seq can measure only the expression of genes in a mixture of different cell types, single-cell RNA-seq (scRNA-Seq) can delineate the expression of different cell types in a complex tissue sample. Theoretically, linking gene expression to specific cells increases the accuracy of measuring by 10x and cuts costs per gigabyte Y G by 76%. O L O HN C Cancerous Tissue E T No expression changes are D RNA Sample N q evident in cancerous tissue A Se - S RNA Expression relative to normal cells. L k O Bul Gene 1 O Gene 2 T C Cancer Normal Gene 3 MI Gene 4 O S I in T gle L - MU Cell R n NA io Cancerous mutations are s s e causing overexpression of r p x “Gene 4” in “Cell Type 3” E Multiple Cell Types Cancer + - + - + - + - Cell Type Cell Type Cell Type Cell Type 1 2 3 4 Sources: ARK Investment Management LLC, 2024. Illustration created with BioRender.com, based on data from Hwang et al. 2018. For informational purposes only and should not be considered investment advice or a recommendation to buy, sell, or hold any particular security. Past performance is not indicative of future results. Forecasts are inherently limited and cannot be relied upon.